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Cryobanking of aquatic species ArchiMer
Martinez-paramo, Sonia; Horvath, Akos; Labbe, Catherine; Zhang, Tiantian; Robles, Vanesa; Herraez, Paz; Suquet, Marc; Adams, Serean; Viveiros, Ana; Tiersch, Terrence R.; Cabrita, Elsa.
This review is focused on the applications of genome cryobanking of aquatic species including freshwater and marine fish, as well as invertebrates. It also reviews the latest advances in cryobanking of model species, widely used by the scientific community worldwide, because of their applications in several fields. The state of the art of cryopreservation of different cellular types (sperm, oocytes, embryos, somatic cells and primordial germ cells or early spermatogonia) is discussed focusing on the advantages and disadvantages of each procedure according to different applications. A special review on the need of standardization of protocols has also been carried out. In summary, this comprehensive review provides information on the practical details of...
Tipo: Text Palavras-chave: Cryobanking; Aquatic animals; Fish gametes; Germplasm.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00341/45172/44567.pdf
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First study in cryopreserved Crassostrea angulata sperm ArchiMer
Riesco, Marta F.; Felix, Francisca; Matias, Domitilia; Joaquim, Sandra; Suquet, Marc; Cabrita, Elsa.
Sperm cryopreservation is a widely employed technique that promotes alternative techniques to contribute to broodstock management or restoration programs for species of commercial interest, endangered species or species with an interesting genotype. The preservation of genetic material from improved stocks or from the original population is extremely important for the oyster aquaculture industry to prevent the potential impacts of epidemic diseases and natural disasters. The Portuguese oyster, Crassostrea angulata, was the most important species commercialized by the shellfish industry. However, inadequate management of this industry and pathology occurrences resulted in a significant decrease in natural populations. For this reason, in this work a...
Tipo: Text Palavras-chave: Crassostrea angulata; Sperm; Cryopreservation; Motility; Viability; Fertilization.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00333/44432/44098.pdf
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Sperm handling in aquatic animals for artificial reproduction ArchiMer
Beirão, José; Boulais, Myrina; Gallego, Victor; O’brien, Justine K.; Peixoto, Silvio; Robeck, Todd R.; Cabrita, Elsa.
Artificial reproduction involves collection and handling of gametes in a way that secures their quality and maximizes the fertilization outcome. In addition to initial sperm quality, numerous steps can affect the final result of fertilization, from the sperm collection process until gamete mixing (or co-incubation) when spermatozoon enters or fuses with the oocyte. In this review, we summarize the whole process of sperm handling, from collection until fertilization for fish, penaeid shrimp, bivalve mollusks and marine mammals. To obtain sperm from captive animals, techniques vary widely across taxa, and include stripping by abdominal massage or testis surgical removal in fish, spermatophore collection in penaeid shrimps, gonadal scarification or...
Tipo: Text Palavras-chave: Sperm extraction; Spermatozoa:oocyte ratio; In vitro fertilization; In vitro reproduction; Assisted reproduction.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00492/60411/63847.pdf
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